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Affidabilità dell'UBC (Urinary Bladder Cancer) antigene come marcatore tumorale vescicale nei pazienti anziani

Francesco Torelli*, Roberto Ricciotti, Riccardo Grifoni, Giovanni Battista Muraro
*Laboratorio di Radioimmunologia - INRCA- (Istituto Nazionale di Ricovero e Cura per Anziani) IRCCS Ancona

Biochimica Clinica: 2002; 27(1): 5-9 [Article in italian]

ABSTRACT. Objective. To evaluate if the tumor marker Urinary Bladder Cancer (UBC) antigen is able to early recognize recurrences of bladder cancer, 25 compared to instrumental investigation. Method. UBC antigen was determined in 273 individuals: 84 apparently healthy subjects, 61 patients affected with benign urologic disorders, 128 patients with histologically proven bladder cancer who previously underwent surgery. UBC antigen was assayed using an immunoradiometric method (IRMA). Results. Data analysis showed a clinical sensivity of 76%, a clinical specificity of 84%, while the diagnostic efficiency resulted 81%. Conclusions. Up to now it is not possible to substitute UBC antigen measurement in urine for cystoscopy in order to diagnose bladder cancer or its recurrences

Approccio multidisciplinare alla diagnosi delle malattie emorragiche congenite

Massimo Franchini*, Giuseppe Lippi
*Servizio di Immunoematologia e Trasfusione, Azienda Ospedaliera di Verona

Biochimica Clinica: 2002; 27(1): 10-15 [Article in italian]

ABSTRACT. Congenital bleeding disorders are puzzling pathologies. Correct and accurate diagnostic and therapeutic approaches require close collaboration among all specialists (laboratory physicians, clinicians, surgeons) involved in the care of these occasionally critical patients, requiring inevitably a multidisciplinary approach. The aim of this review is to analyze briefly the major hereditary bleeding disorders (hemophilia A, B, C and von Willebrand disease), providing a systematic approach to the bleeding patient. Finally, we focus on some important preanalitycal factors (age, sex, hormonal status, blood type, phlogistic reactions), influencing the coagulation system.

Analisi della distribuzione dei valori di TSH su dried-spot nello screening neonatale per ipotiroidismo congenito

Marco B.L. Rocchi*, Renzo Ciatti, Giulia Burroni, Silvia Bellagamba, Vera Stoppioni, Massimo Burroni
*Istituto di Biomatematica, Facoltà di Farmacia, Università di Urbino "Carlo Bo

Biochimica Clinica: 2002; 27(1): 16-19 [Article in italian]

ABSTRACT. In this paper, some different statistical methods for the identification of the cut-off value of TSH on dried-spot, for the recall of the newborns in the neonatal screening for Congenital Hypothyroidism are evaluated and compared. Data were obtained from the Neonatal Screening Register of Regione Marche, Italy, set at the U.O. Neuropsichiatria Infantile of the Fano Hospital. The results indicate that, at the present, the non parametric method of Lee Herrera, based on the percentiles, appears the most reasonable approach in order to deal with this problem.

Elettroforesi capillare: confronto quantitativo delle zone albumina e a-1-globulina con le corrispondenti proteine specifiche

Paola Luraschi*, Simona Brambilla, Ilenia Infusino, Anna Pagani, Carlo Franzini
*Ospedale L. Sacco, Laboratorio di Chimica Clinica, Milano, Italy

Biochimica Clinica: 2002; 27(1): 20-24 [Article in italian]

ABSTRACT. Due to measurement by UV absorption, capillary zone electrophoresis permits reliable quantitation of the separated zones. It is therefore important that detailed information on the relationship among the zones and the relevant protein species is available. We compared the albumin and a-1-globulin zones electrophoretic with respectively the albumin and the sum (a-1-antitrypsin + a-1-acid glycoprotein) measured with alternative methods. Two sets of fresh patient sera (n = 188 and n = 114), selected to cover wide intervals of zone values, were used. Electrophoretic zones were measured with a Beckman Coulter Paragon CZE 2000 system. Specific proteins were measured by immunonephelometry (albumin, a-1-antitrypsin and a-1-acid glycoprotein) and by dye-binding (albumin). CZE albumin compared favourably with immunochemical albumin (mean difference: 2.6 ± 2.21 g/L) and with dye-binding albumin (mean difference: -0.80 ± 2.00): the observed differences were lower than the biological variation-based critical difference (11%) in the albumin concentration interval 25.0-60.0 g/L. The CZE a-1-globulin zone correlated significantly with the sum (a-1-antitrypsin + a-1-acid glycoprotein), but showed to be significantly higher (mean difference: 2.38 ± 0.72 g/L).We conclude that CZE albumin values are clinically equivalent to alternative albumin measurements in the mentioned concentration interval. CZE a-1 zone gives information about increases of the two major acute phase proteins comparable to specific protein measurements; however, additional UV-absorbing material contributes significantly to the CZE zone.

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