A B S T R A C T S   N U M E R O   2 / 1999

Effect of excessive salt intake on renal function in sodium-sensitive young adults

A.A. Fasanmade*, J.L. Fajimi
*Departments of Physiology and Chemical Pathology College of Medicine University of Ibadan Ibadan, Nigeria

Biochimica Clinica: 1999; 23(2):65-67 [Article in english]

ABSTRACT. Creatinine clearance of normotensive subjects were determined to assess the role of glomerular function in the elevation of blood pressure exhibited by sodium sensitive subjects during periods of excessive salt intake. 24 normotensive male medical students, 7 of whom are sodium sensitive, were each given oral supplemental salt, equivalent to 200mmol. Na+ daily for one week. Blood pressure response was monitored along with creatinine levels in venous blood and 24-h urine samples. Blood pressure increased significantly in the sodium sensitive subjects but there was no significant difference in creatinine clearance following excessive salt intake in both groups. It is unlikely, therefore, that glomerular dysfunction plays a major role in the elevation of blood pressure response to excessive salt intake by normotensive sodium sensitive subjects.

Dosage of % Carbohydrate deficient transferrin (%CDT): a preco-ciuous biological marker of alcoholism easily determinable

L. Reccia*, M. Lioniello, R. Morelli, G. Tudisca, G.G. Di Costanzo
*Laboratorio Tossicologico, A.S.L. Napoli 3

Biochimica Clinica: 1999;23(2):68-74 [Article in italian]

ABSTRACT. Aim of our work was to evaluate the sensitivity and specificity of dosage of %CDT in 69 subjects: 19 alcoholics without hepatic disease (GROUP 1), 21 alcoholics with hepatic disease (GROUP 2) and 29 normal subjects (GROUP 3). The mean value of %CDT was 7,76ć1,96 in the GROUP 1, 9,23ć2,51 in the GROUP 2, 2,69ć0,70 in the GROUP 3. In the GROUP 1 sensibility was 84,2%, in the GROUP 2 85,7%; the global sensibility was 85% with a global accuracy of 92,75%. Specificity was 100%. T-Student test, used to con-front the mean value of %CDT of GROUP 1 nei confronti del GROUP 2, showed a difference statistically significative (p < 0,005); also the confront of mean value of %CDT of GROUP 1 + 2 nei con-fronti del GROUP 3 was significative (p < 0.0001).

Circulating fetal cells in maternal blood detected by flow cytometry

F. Ferrara*, E. Makovec, G. Passerini, G. Banfi, P.A. Bonini
*Servizio Integrato di Medicina di Laboratorio - IRCCS H S. Raffaele - Via Olgettina, 60 - 20132 MILANO

Biochimica Clinica: 1999;23(2):75-80 [Article in italian]

ABSTRACT. The detection of circulating fetal cells in maternal blood is usually performed using the slide-based microscopic counting method of acid elution also called Kleinhauer-Betke test: this method show strong limitation in precision and sensitivity. This report illustrates a rapid flow cytometric assay for fetal erythrocytes identification, based upon HbF (hemoglobin F) content, using anti HbF fluorescein isothiocyanate labeled monoclonal antibody. The technology of flow cytometry, capable of both high sensitivity of detection and rapid cell-by-cell analysis, seems ideally suited for such an application. In our study, the precision of the method was satisfactory: within-run CV (Coefficient of Variation) was 16.14% (Standard deviation, SD = 0.402), whilst the between-run CV was 14.71% (SD = 0.174). Comparison of data obtained from 30 venous blood amples show a good correlation between flow cytometry and HPLC (r = 0.97; y = 1.03 x + 0.091).

Circadian rhythm in elimination of saliva Secretory IgA - a case study

F. Rosaschino*, G. Cisarri, C. Maggioni, G. Corti, R. Torre
*Institute of Pediatrics-University of Milano Insitute of obstetric and gynecology-I Clinic-University of Milano and Istituti Clinici di Perfezionamento-Milano

Biochimica Clinica: 1999;23(2):81-88 [Article in italian]

ABSTRACT. The salivary excretion of secretory IgA has been studied in a healthy male child, aged 3 a half year at the beginning of the study. The study has been repeated on 5 different occasions, during a two years time span. The occurrence of a significant circadian rythm, constant and stable during the whole study duration, has been shown, with acrophase around 02:00 and 04:00 am. The underlaying regulation mechanisms for such a rythm, and the possible relationships between blood IgA and salivary secretory IgA are discussed.

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