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Valutazione clinica e di laboratorio di un metodo ELISA con antigene umano ricombinante per la determinaizone degli anticorpi anti-Transglutaminasi

Mara De Amici*, Maurizio Perego, Cristina Torre, Vincenzo Cinapri, Francesca Torello, Katerina Vjero, Remigio Moratti
*Dipartimento di Pediatria, IRCCS Policlinico S. Matteo Pavia Università degli Studi di Pavia

Biochimica Clinica: 2003; 27(4): 215-217 [Article in italian]

ABSTRACT. The availability of new ELISA tests that uses human recombinant tissue transglutaminase opens new diagnostic opportunities. The aim of the study has been to evaluate the new analytic test that employed recombinant human antigen for the dosage of anti-tranglutaminase antibodies, with respect to guinea pig transglutaminase ELISA. Our results show a good sensibility and a high specificity, mainly for the test with recombinant human tTG, and suggest to utilise the test for the screening of patients with suspected coeliac disease.

Iperfosfatasemia ereditaria benigna di origine intestinale: report di due casi

Sandra Ratibondi, Silvio Mosconi, Andrea Perina, Barbara Betemps Alessandra Fosson, Francesco Di Vito
*Laboratorio Analisi Chimico-Cliniche, Ospedale Regionale della Valle d'Aosta

Biochimica Clinica: 2003; 27(4): 218-221 [Article in italian]

ABSTRACT. Two subjects with benign hyperphosphatasemia of intestinal origin were studied; we have found no evidence of clinical abnormalities or other explanations. Alkaline phophatase (ALP, EC 3.1.3.1) isoenzymes were determined with Helena-REP System and determination of total ALP activity was executed automatically on Hitachi 747 with optimized standard method of German Society of Clinical Chemistry. Agarose gel electrophoresis of ALP in serum showed markedly increased intestinal isoforms in both cases; we have also tried to describe the family pedigree of the patients. In the first case, although clinical data suggested a pathological significance for the increase of intestinal ALP, this conclusion was later excluded. In the second case, the description of the family suggested genetic inheritance of this condition. Exact recognition of this benign biochemical abnormality should help in avoidlng unnecessary furhter investigation.

Automazione in allergologia: valutazione del sistema ADVIA Centaur nella determinazione delle IgE specifiche del siero

Eliana Baghino, Eugenia Bovina, Gaetano Magrì*, G. Domenico Poggi, Anna Tinivella, Giuseppe Ricchetti, Gianluca Garello
*Laboratorio Analisi, Ospedale di Imperia (Direttore Dr.Gian Domenico Poggi), Ospedale di Galliate (Direttore Dr.ssa Anna Tinivella)
Biochimica Clinica: 2003; 27(4): 222-224 [Article in italian]

ABSTRACT. The availability of new automatic systems allows the laboratories involved in immunochemical measurements to consolidate immnochemical tests and specific IgE measurements on the same analytic platform. This work's aim was to verify if such a technological innovation allows specif serum IgE to be measured with the accuracy level required by allergologic diagnostics. To this aim we have compared the analytical results from a new system, the ADVIA Centaur (Bayer), with those from an accepted and largely used alternative system, the UniCAP (Pharmacia).

Antigene prostata-specifico totale, libero e complessato: relazioni tra le differenti misure

Luisa Scapellato*, Anna Pagani, Carlo Franzini
*Laboratorio di Analisi Chimico Cliniche, Ospedale L. Sacco, Milano
Biochimica Clinica: 2003; 27(4): 225-229 [Article in italian]

ABSTRACT. Measurement of the free portion of serum prostate spcific antigen (fPAS), generally reported as free to total PSA ratio (f/t PSA), is widely used for modulating the medical decision in the presence of moderately increased levels of total serum PSA. Alternatively, the complexed portion of serum PSA (cPSA) can be measured. In order to evaluate possible differences in patients' classification resulting from the two different measurements, we assayed fPSA, cPSA and tPSA in 298 serum samples selected indipendently from the clinical diagnosis, covering the 2.0-25.9 ng/mL interval of total PSA concentration. 206 of such samples had PSA levels in the "grey-zone" interval (4.0-10.0 ng/mL). The sum (fPSA + cPSA) agreed with measured tPSA values, both in the whole group (n=298) and in the grey-zone subgroup of sera (n=206), pointing out at equimolarity of measurements. The f/t PSA ratios were caculated either from fPSA and tPSA results or from cPSA and tPSA results. In the grey-zone subgroup the two sets of calculated ratios agreed: taking 0.20 as the ratio's cut-off value for the ratio, patient's classification agreed in 168 (82%) case and disagreed in 38 (18%) cases. In most cases, however, different classification was due to small variations among the ratio values.

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