A B S T R A C T S   N U M E R O   6 / 2 0 0 0

Valutazione multicentrica di cinque metodi per la determinazione della mioglobina del siero*

Sara Altinier, Paolo Amboni, Roberto Bonora, Antonio Brustia, Alberto Dolci, Andrea Motta, Franca Pagani, Patrizia Pergolini, Arialdo Vernocchi, Martina Zaninotto, Mario Plebani, Mauro Panteghini* a nome del Gruppo di Studio Intersocietario 'Marcatori di Lesione Miocardica' della Società Italiana di Biochimica Clinica e Biologia Molecolare Clinica (SIBioC) e della Società Italiana di Medicina di Laboratorio (SIMeL)
*Laboratorio Analisi Chimico Cliniche 1, Azienda Ospedaliera Spedali Civili, Brescia

Biochimica Clinica: 2000; 24(6): 461-468 [Article in italian]

ABSTRACT. A multicenter study was carried out to compare the analytical performance of five commercially available assays for myoglobin measurement. Linearity, imprecision, interferences, and method comparison were studied according to NCCLS guidelines, whereas reference values were determined following IFCC recommendations. BNA and Opus assays showed relatively high imprecision (all but one total CVs >7.4%). Other assays showed lower CVs, that varied, however, among laboratories, particularly at a normal myoglobin concentration (Access, 6.0 vs 10.9%; Hitachi, 3.8 vs 5.8%; Stratus, 3.4 vs 6.5%). Results were lower in anticoagulated samples on Access, in heparin and citrate samples on Stratus and in citrate samples on BNA and Opus, and increased on Hitachi with heparin and EDTA. Use of separator gel produced results significantly lower (P <0.001) on Hitachi and higher (P = 0.016) on Opus. Bilirubin, turbidity, and hemoglobin had no effect on evaluated methods, but rheumatoid factor affected the Access. In method comparison, high correlation coefficients (ō0.98) were obtained. However, Stratus gave higher results, whereas Access and BNA gave the lowest. The following upper reference limits (mg/L) for men and women were obtained: Access, 70 and 52; BNA, 51 and 49; Hitachi, 67 and 58; Opus, 80 and 50; Stratus, 86 and 63. The possibility of high imprecision and marked disagreement among commercial myoglobin assays should be carefully considered in clinical practice.

I protocolli biochimici per la diagnostica cardiaca nell'era delle troponine: verifica dell'utilizzo di alcune mappe di cura ad un anno dalla loro introduzione

Mauro Panteghini*, Franca Pagani, Graziella Bonetti, Claudio Cuccia
*Laboratorio Analisi Chimico Cliniche 1, Spedali Civili, Brescia

Biochimica Clinica: 2000; 24(6): 469-475 [Article in italian]

ABSTRACT. Previously in this journal, we described the development and the implementation of new biochemical strategies for diagnosing and monitoring patients with acute coronary syndrome in our clinical practice. The main goals of these care maps, containing the definition of the optimal sequence of acts and interventions, were the delay reduction and the elimination of unjustifiable differences in the diagnostic approach and management of these patients. After one year from their introduction, we report here the audit's findings. Our experience show that the collaboration and cooperation between those with expertise in cardiology, laboratory medicine, and quality improvement, working inside the hospital, may permit to achieve the goals reported above, through a continuous improvement of the processes and the introduction of changes directed to further improve the obtained results as well.

La renina attiva nell'infarto acuto del miocardio

Piernatale Lucia*, Stefania Caiola, Alessandro Coppola, Alessandra Bocciarelli, Massimo Trappolini, Luca L. Manetti, Ettore Maroccia, Angela M. Buongiorno, Carlo De Martinis
*II Clinica Medica, Universitą 'La Sapienza' Roma

Biochimica Clinica: 2000; 24(6): 476-488 [Article in italian]

ABSTRACT. Plasma renin activity (PRA) is increased in acute myocardial infarction (AMI). However, PRA could be under- or overestimated with respect to the active renin (AR) values. The aim of our study is to investigate the variability of AR in the first two weeks of AMI course in different subgroups of patients. The circulating levels of AR are significantly higher than normal within the first 6 hours from the beginning of symptoms untill the covered period, in spite of the large variability according to the investigated subgroup. The highest concentration is found in patients who die during the in-hospital period and in those with adverse prognostic factors (aged subjects, anterior or anterolateral AMI, heart failure, not fibrinolysed). These findings suggest that AR can be regarded as an useful prognostic index in the acute phase of myocardial infarction.

Comparison among bicarbonate and base excess values as obtained from NCCLS standard C12-A equations and from equations suggested by other authors

Stefania Volonté*, Lorenzo Stangoni, Antonella Bertoncello, Mariapia Cantaluppi, Enrica Corvi, Natalina Lacidogna, Giovanbattista Orsini, Valter Gualandri
*Laboratory of Clinical Chemistry, Casa di Cura S. Pio X - Milan Italy

Biochimica Clinica: 2000; 24(6): 489-492 [Article in english]

ABSTRACT. This article reports a compared evaluation of NCCLS standard C12-A equations for bicarbonate and base excess (BEb) withother algorithms suggested by other authors and used by different emogas analyzers.

Torna al Numero 05/2000 | Vai all'Indice di Biochimica Clinica | Vai al Numero 1/2001

Per chi desidera pubblicare su BC, sono disponibili le Istruzioni per gli Autori
Per chi desidera sottoscrivere l'abbonamento a BC, è disponibile la Cedola di Registrazione