A B S T R A C T S   N U M E R O   9 / 1998

Evaluation of the PHOTOFIX system, a new techique for proteins identification by.

*Rovescala M, Ciaiolo C.
*Laboratorio di Analisi Chimico-Cliniche e Microbiologia - Ospedale Civile, 27049 Stradella (PV)
Dipartimento di Medicina ed Oncologia Sperimentale, Sezione di Ematologia, Università degli studi di Torino, 10100 Torino (TO).

Biochimica Clinica 1998; 22(9): 493-495 [Article in italian]

ABSTRACT. According the age of the patients, Monoclonal Component (CM) can be obeserved with a frequency ranging from 2/1000 to 75/1000. Immunofixation electrophoresis (IFE) is the most employed method to recognize monoclonal immunoglobulins. The PHOTOFIX system is an innovative procedure for MC immunotyping; in October 1996 it was presented at the XXVIII SIBioC National Congres. To evaluate the advantage of this new method we assayed 40 serum samples with one or more MC and selected on the basis of their MC concentration, varying from low (not detectable by densitometry) to very high (60 g/L). The detection limits for IgG, IgA, IgM, k and light chain, was assessed using diluitions of a pool of sera from healthy peoples. The PHOTOFIX system show the same results as those obtained with traditional IFE, bat a more simple, faster and clearer method furnishing also a good photographic file.

Standardization of biochemical markers of cardiac damage: problems and review of the international activities.

*Bonetti G., Pagani F., Panteghini M.
*I Laboratorio Analisi Chimico-Cliniche, Spedali Civili, 25123 Brescia

Biochimica Clinica 1998; 22(9): 496-502 [Article in italian]

ABSTRACT. The field of biochemical markers of cardiac damage is in a dynamic state, with new applications continually appearing and new assays and markers being developed. These significant and sudden advancements in the development of new biochemical assays have led however to several analytical and interpretative problems. In this situation, it is essential that a uniform and rigorous outlook be mantained to ensure optimal test utilization through a coordination of all standardization efforts. In this presentation, we review the main problems and list the national and international activities devoted to resolve them.

Endothelins and human pathologies.

*Perilli E., Catalano M.
*Centro di Ricerca per la Prevenzione e la Patologia Vascolare Periferica, Università degli Studi di Milano, Ospedale L. Sacco, Milano

Biochimica Clinica 1998; 22(9): 503-515 [Article in italian]

ABSTRACT. Endothelins are substances produced by the endothelium that have a vasoconstrictive effect. From a functional point of view, endothelins can be considered the physiological antagonists of various different substances that have a vasodilatative effect, and they contribute, together with the latter, towards cotrolling the vascular tone. Interest in these sustances has further increased following the discovery of their involvement in various different diseases in man. This review considers first of all the structure, biosynthesis and activity of endothelins. Then the role of these substances in hypertension and other diseases, including atherosclerosis, cerebrovascular diseases, peripheral vascular disease and other pathological conditions linked with hypertension is discussed. In the conclusion, the widespread nature of the action of endothelins in many tissues and in a wide range of pathological conditions is mentioned.

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